| |
Presented at the NABS Annual meeting, Athens, Georgia, 2003
in Molecular Approaches to Population Structure and Bioassessment
Using DNA markers to invesitgate interbasin relationships and define species classification of stoneflies.
J.S.K. Kauwe1, D.K. Shiozawa1,2, and R.P. Evans1. 1Department of Integrative Biology, Brigham Young University, Provo, UT 84602, 2Monte L.Bean Life Science Museum, Brigham Young University, Provo, UT 84602
Phylogenetic data provides insight into the evolutionary and dispersal history of organisms; these processes are directly related to geological history. In the case of aquatic species, this link is especially strong. We used over 800 base pairs of the mitochondrial gene cytochrome B to investigate the phylogeography of the stoneflies Pteronarcys californica and Pteronarcys princeps. Sequences were obtained from over 200 individuals from 39 different populations in the Western United States. While intra-population variation was minimal, inter-population variation provided useful phylogenetic signal in both species. These data clearly define the two species, and suggest that the population structure of P. princeps is more fragmented than that of P. californica; possibly indicating that it is older in origin, or invaded this region earlier. The resulting phylogeny was compared to known geological history.
|
|
