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Presented at the NABS Annual meeting, Pittsburgh, Pennsylvania, 2002
in Ecotoxicology
ENZYME-LINKED IMMUNOSORBENT ASSAY OF CHLORPYRIFOS AND ATRAZINE IN NATIVE AND EXOTIC CRAYFISH FROM LANCASTER COUNTY, PA.
J.A. Iera1, S.L. Turchi1, and J.R. Wallace2. 1Department of Chemistry, Millersville University, Millersville, PA 17551, 2Department of Biology, Millersville University, Millersville, PA 17551
Our objective in this study was to assay for the presence of two pesticides, atrazine and chlorpyrifos, in crayfish collected from creeks and rivers throughout Lancaster County, Pennsylvania. Approximately 100 crayfish from 33 different sites were analyzed. Enzyme-linked immunosorbent assays (ELISA) were used to detect the presence of the pesticides in the tissue of the crayfish. Both pesticides were detected in the entire set of samples. A maximum concentration of chlorpyrifos at 4.6 ppb and a maximum concentration of atrazine at 3.4 ppb occurred in crayfish from the same site in the county, and from the same crayfish species Orconectes limosus. The tests conclude that chlorpyrifos and atrazine exist in crayfish throughout Lancaster County, which leads to an environmental concern of the area, as well as a concern of the pesticide levels that may exist in crayfish sold as live bait.
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