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Presented at the NABS Annual meeting, Vancouver, British Columbia, 2004
in Systematics 1
Cambarus dubius Faxon 1884 (Decapoda: Cambaridae), one species or several? Morphology, color patterns, geography, and genetics: combining tools to explore a century of taxonomic debate.
N.L. Smith1, F.E. Anderson1, and G.A. Schuster2. 1Department of Zoology, Southern Illinois University, Carbondale, IL 62901, 2Department of Biological Sciences, Eastern Kentucky University, Richmond, KY 40475
Cambarus (Jugicambarus) dubius Faxon 1884 is a primary burrowing crayfish that has several color morphs through out its range. These color morphs, along with the high level of morphological variation within the species, have lead to suggestions that there may be several species or sub species all currently identified as Cambarus dubius. Genetic diversity within C. dubius could be structured by color (i.e., each color morph is a reproductively isolated unit or monophyletic group), by geography (i.e., closely related individuals are geographically close to one another) or some combination of the two. To test these hypotheses, C. dubius specimens were collected from Kentucky, North Carolina, Tennessee, and West Virginia. The color pattern and collection locality of every individual was recorded. Standard morphological measurements were taken on freshly collected specimens and on specimens from museum collections. These data were analyzed using principal components analysis (PCA). A ~650bp fragment of the mtDNA COI gene was sequenced from several individuals and the sequences were analyzed in PAUP*. Correlations between the resulting phylogeny and both color pattern and geographic distribution were investigated.
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